TABLE 4.
Effect of PMN depletion on survival timea
| Treatment | Survival time (h)
|
|
|---|---|---|
| Mean ± SD | Median | |
| Saline + control IgG | 123 ± 22 | 115 |
| Saline + anti-Ly-6G IgG | 47 ± 3 | 47 |
| LPS + control IgG | 191 ± 60 | 168b |
| LPS + anti-Ly-6G IgG | 73 ± 3 | 74c |
a
Mice were pretreated i.v. with saline or serovar Abortus equi LPS (1 mg/kg) three times at 24-h intervals. Twenty-four hours after the last LPS injection, LPS-tolerant mice and saline-pretreated controls were infected with serovar Typhimurium (106 bacteria/kg i.p.), and survival time was determined. To study the role of PMNs, the mice received anti-Ly-6G IgG (RB6-8C5) or normal rat IgG i.p. 16 h prior to (0.6 mg) and 8 and 32 h after (0.3 mg) initiation of infection. The data are from one of two experiments (n = 6).
b
Significantly different from saline plus control IgG (P < 0.05).
c
Significantly different from saline plus anti-Ly-6G (P < 0.001).