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. 2001 Jan;69(1):479–485. doi: 10.1128/IAI.69.1.479-485.2001

FIG. 8.

FIG. 8

Incorporation of radiolabeled amino acid into CD14. (A) HUVEC (2.5 × 106/assay) were incubated for 24 h with l-[35S]methionine, and the extent of incorporation into CD14 was determined by immunoprecipitation. The results of three experiments, each with cells from a different cord, are expressed as counts per minute (counts per minute values of isotype-matched controls, 1,400 for AB serum and 2,300 for FCS, have been subtracted). (B) The SDS-PAGE profile of the immunoprecipitates from l-[35S]methionine-labeled endothelial cell lysates confirmed their molecular mass to be ∼55 kDa. Statistical analysis: ∗, FCS-stimulated cells versus unstimulated cells, P < 0.0001.