Fig. 1.

Validation of primary HESC in vitro decidualization. HESC were cultivated for 6 days in 2%-FBS medium containing MPA, E, and 8-Br-cAMP (D cells) or in 2%FBS medium containing vehicle solutions (ND cells). a PRL levels in cell culture supernatant. Data represents mean mU/l ± SEM (n = 5). b G6PD, GLUT1, and GLUT3 mRNA expression levels. Data represent mean fold change compared to ND cells (dotted line, fold change = 1.0) ± SEM (n = 5). *p < 0.05 and ***p < 0.001 ns, non-significant (compared to ND cells). HESC, human endometrial stromal cells; D, decidualizing; ND, non-decidualizing; MPA, medroxyprogesterone acetate; E, estradiol; 8-Br-cAMP, 8-bromo-cyclic adenosine monophosphate; PRL, Prolactin; GLUT, glucose transporter; and G6PD, glucose-6-phosphate dehydrogenase