Fig. 3.

In vitro decidualization of HESC modifies the expression levels of enzymes associated with pyruvic and lactic acid metabolism. HESC were cultivated for 6 days in 2%-FBS medium containing MPA, E, and 8-Br-cAMP (D cells) or in 2%FBS medium containing vehicle solutions (ND cells). a PDHA1 and LDHA mRNA expression levels. Data represent mean fold change compared to ND cells (dotted line, fold change = 1.0) ± SEM (n = 5). b Lactic and pyruvic acid levels. Data represent mean μmol/g of total protein (n = 4). **p < 0.01 (compared to ND cells). HESC, human endometrial stromal cells; D, decidualizing; ND, non-decidualizing; MPA, medroxyprogesterone acetate; E, estradiol; 8-Br-cAMP, 8-bromo-cyclic adenosine monophosphate; LDH, lactate dehydrogenase; and PDH, pyruvate dehydrogenase