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. 2022 Dec 12;15:1068164. doi: 10.3389/fnmol.2022.1068164

Figure 3.

Figure 3

Knocking of C/EBPβ alleviates HFD-induced depression-like behaviors. (A) Schematic of the HFD or chow diet course and behavioral tests process. HFD, high-fat diet; TST, tail suspension test; FST, forced swim test; SPT, sucrose preference test; d, day. (B) Hippocampal CEBPB mRNA levels in 8–10 week-old wild-type (C/EBPβ +/+) and C/EBPβ +/− male mice fed with HFD or chow diet for 12 weeks. Data represent the mean ± SEM of six samples per group (**p < 0.01; NS, not significant; one-way ANOVA and Bonferroni’s multiple comparison test). (C) Hippocampal BDNF mRNA levels. Data represent the mean ± SEM of six samples per group (*p < 0.05; NS, not significant; one-way ANOVA and Bonferroni’s multiple comparison test). (D,E) ELISA quantification of (D) TNFα, IL-1β, and IL-6 and (E) BDNF in the brain lysates from the above mice. Data in (D,E) represent the mean ± SEM of six samples per group (*p < 0.05, **p < 0.01; NS, not significant; one-way ANOVA and Bonferroni’s multiple comparison test). (F) Representative immunoblots and (G) quantification of hippocampal p-C/EBPβ, C/EBPβ, and BDNF protein expression. Data in (F) are representative of three independent experiments. Data in (G) represent the mean ± SEM (n = 6 for each group, *p < 0.05, **p < 0.01; NS, not significant; one-way ANOVA and Bonferroni’s multiple comparison test). (H) Tail suspension test, (I) forced swim test and (J,K) sucrose preference test for the above mice. Data represent the mean ± SEM (n = 10 mice for each group; *p < 0.05; NS, not significant; one-way ANOVA and Bonferroni’s multiple comparison test).