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. 2022 Dec 12;9:1069797. doi: 10.3389/fnut.2022.1069797

FIGURE 5.

FIGURE 5

Identification and miRNA expression level of EVs and dual-luciferase reporter assay analysis. (A) Serum EVs of different groups observed by transmission electron microscopy (TEM) at the scale of 200 nm. (B) Particle size analysis showing that the particle size of each exosome ranged from 50 to 140 nm. (C) Expression levels of CD9, CD63, and TSG101 determined by Western blot. (D) miRNAs were extracted from rat serum EVs, and their expression levels were detected by qRT-PCR. Significant difference was indicated with the sign (*P < 0.05) and (**P < 0.01) vs. HFD group; (#P < 0.05) and (##P < 0.01) vs. HFD + NFEG group. Dual-luciferase reporter assay analysis: (E) the top portion of panel (E) shows the binding site of miR-126a-5p mimics and corresponding target genes HMGCR, and the bottom portion of panel (E) shows the relative fluorescence values after transfection and mutation, which suggested that significant interaction existed between miR-126a-5p and target gene HMGCR. Panel (F) shows similar results on the interaction of miR-30b-5p with MTP. Significant difference was indicated with the sign *P < 0.05.