FIG. 8.

Upregulation of CD54 on immature DCs stimulated with either T. pallidum or the synthetic lipopeptide. XS52 cells were stimulated for 24 h, stained with a FITC-conjugated anti-CD54 monoclonal antibody, and then analyzed by flow cytometry. (A) LPS (40 ng/ml) was included as a positive control. (B) T. pallidum (Tp) (1,000 spirochetes/cell), but not TEx, induced CD54. (C) The synthetic lipopeptide (47-L) (32 μg/ml), but not the 47 hexapeptide control (47-HX), induced CD54. NA, no application (unstimulated cells). Results are representative of two independent experiments.