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. 2022 Dec 12;13:1001778. doi: 10.3389/fmicb.2022.1001778

Figure 3.

Figure 3

YLZD upregulated the expression levels of hepatic SIRT1/Nrf2 pathway-related genes and proteins. (A) Typical IHC staining images of the liver with different treatments (magnification, ×200). (B,C) Average optical density values (AOD = IOD/Area) of SIRT1 and Nrf2 protein expression. (D) Expression of transcription factors associated with SIRT1/Nrf2 pathway were detected by Western blot. (E,F) Quantification of the corresponding Western blotting results in D. (G–J) RT-qPCR of relative mRNA expression of SIRT1, Nrf2, HO-1 and NQO1 in liver. Values are represented as mean ± SEM (n = 6 rats/group). ##p < 0.01, #p < 0.05 versus the Control group; **p < 0.01, *p < 0.05 versus the HFD group.