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. 2022 Dec 12;13:1001778. doi: 10.3389/fmicb.2022.1001778

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Copyright © 2022 Jiang, Mao, Sun, Shi, Han, Zhang, Zhang, Wang, Hu, Zhang, Li and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of acetic acid and butyric acid on cell viability and FFA-induced lipid accumulation and oxidative stress in L02 cells. (A,B) The effects of AA and BA on L02 cell viability performed by CCK8 assays. (C) Cell TG level. (D) Cell Oil red O staining (magnification, ×400). (E) Quantitative analysis of oil red staining results was performed using Image Pro Plus 6.0 software. (F–H) The level of cell MDA, SOD and GSH-Px, respectively. L02 cells were pre-treated for 24 h with 1.0 mM FFA to constructed NAFLD cell model before treated with 1.0 mM AA or 0.5 mM BA for another 24 h. Data represent means ± SEM. ^##p < 0.01 versus Control; ^*p < 0.05, ^**p < 0.01 versus FFA.