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. 2022 Dec 12;13:1001778. doi: 10.3389/fmicb.2022.1001778

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Copyright © 2022 Jiang, Mao, Sun, Shi, Han, Zhang, Zhang, Wang, Hu, Zhang, Li and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Butyric acid ameliorated FFA-induced L02 cells oxidative stress injury via the SIRT1/Nrf2 pathway. (A) Cell Oil red O staining (magnification, ×400). (B) Quantitative analysis of oil red staining results was performed using Image Pro Plus 6.0 software. (C) Cell TG level. (D–F) Western blot analysis of SIRT1 and Nrf2 in L02 cells. (G–I) The level of cell SOD, GSH-Px and MDA, respectively. (J,K) Histogram of intracellular ROS level detected by DCFH-DA. L02 cells were pre-treated for 24 h with 1.0 mM FFA to constructed NAFLD cell model before treated with 0.5 mM BA or 100 nM EX-527 for another 24 h. Data represent means ± SEM. ^##p < 0.01 versus Control; ^*p < 0.05, ^**p < 0.01 versus FFA.