Skip to main content
. 2022 Dec 15;87:104406. doi: 10.1016/j.ebiom.2022.104406

Fig. 5.

Fig. 5

Luteolin treatment improves remyelination and Kir4.1 expression in tMCAO mice. (a and b) Representative electron micrographs show that the presence of impaired axons with demyelination in ipsilateral cortex compared with its contralateral region at day 9 after tMCAO in wild type mice saline group at postnatal 8 weeks (a) and the remyelinated axons in ipsilateral cortex compared with its contralateral region at day 9 after tMCAO in wild type mice after luteolin treatment at postnatal 8 weeks (b). Scale bars: 2 μM. (c and d) The percentage of healthy axons (the normal diameter of myelinated axons is over 200 nm) between contralateral and ipsilateral cortex after 9 days tMCAO in saline and luteolin group. n indicates the number of axons. The data were normally distributed and statistical significance was assessed within each group using two-tailed unpaired t-test. ∗∗p < 0.01; ∗∗∗p < 0.001, n.s indicates not significant. (e) The box-plots represent average of myelin sheath thickness between saline group and luteolin treatment group after 9 days tMCAO. The data were normally distributed and statistical significance was assessed within each group using two-tailed unpaired t-test. ∗∗∗p < 0.001, n.s indicates not significant. The analyzed axons are from 6 mice per group. (f) The box-plots represent average G-ratio of myelinated axons between saline group and luteolin treatment group after 9 days tMCAO. The data were normally distributed and statistical significance was assessed within each group using two-tailed unpaired t-test. ∗∗∗p < 0.001, n.s indicates not significant. The analyzed axons are from 6 mice per group. (g) Representative images of Kir4.1 and MBP protein expression in sham and tMCAO mice (contralateral and ipsilateral sides) after 9 days luteolin treatment. Note that both Kir4.1 and MBP expressions are significantly increased in ipsilateral sides after luteolin administration in tMCAO mice compared with its saline group. (h) Summary graphs show the average of Kir4.1 and MBP expressions by Western blot in sham mice and both contralateral and ipsilateral sides of tMCAO mice after 9 days luteolin treatment. ∗∗p < 0.01, n.s indicates not significant, n = 3 mice for both saline and luteolin group in sham control; n = 6 mice for both saline and luteolin group in contralateral side after tMCAO; n = 8 mice for both saline and luteolin group in ipsilateral side after tMCAO, one-way analysis of variance (ANOVA) followed by Bonferroni post-hoc tests.