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. 2022 Dec 12;10:1066504. doi: 10.3389/fcell.2022.1066504

FIGURE 1.

FIGURE 1

GARP deficiency affects Golgi structure. (A) Airyscan microscopy of VPS53KO, VPS53KO R, VPS54KO, and VPS54KO R cells stained for trans-Golgi Rab6 and cis-Golgi GM130. Individual channels are presented as black and white images, while overlays are presented as RGB images. (B) Quantification of trans-Golgi area. (C) Quantification of cis-Golgi area. n = 30 cells per group. (D) Airyscan microscopy of Nocodazole treated cells, stained for trans-Golgi Rab6 and cis-Golgi GM130. (E) Quantification of the distance between the cis- (GM130) and trans- (Rab6) Golgi compartments. (F) Transmission electron microscopy of WT, VPS53KO, and VPS54KO RPE1 cells. (G) Quantification of normal and abnormal (swollen or fragmented) Golgi phenotypes in WT, VPS53KO, and VPS54KO cells. n = 30 cells were imaged per group for the quantification. Statistical significance was calculated using one-way ANOVA in GraphPad prism. **** p ≤ 0.0001.