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BIG1 is mislocalized to endolysosomal compartment in GARP-KOs. Airyscan microscopy of VPS54KO and VPS54KO R cells co-stained for BIG1 and ERGIC-53 (A), BIG1 and SNX1 (C), BIG1 and LAMP2 (E), BIG1 and CD63 (G). The small square box in (E) left and right panel indicates enlarged images. Colocalization analysis between BIG1 and ERGIC-53 (B), BIG1 and SNX1 (D), BIG1 and LAMP2 (F), BIG1 and CD63 (H) by Pearson’s correlation coefficient analysis. n = 30 cells. **** p ≤ 0.0001, ** p ≤ 0.01.
