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. 2022 Dec 12;10:1066504. doi: 10.3389/fcell.2022.1066504

FIGURE 5.

FIGURE 5

GARP-KO is more detrimental to Golgi residents than deletion of Golgi v-SNAREs. (A) Testing of GOSR1KO and BET1LKO by WB analysis. (B) Total cellular lysates were prepared from RPE1 WT, VPS54KO, BET1LKO, and GOSR1KO cells and total protein abundance was analyzed by WB for SDF4, (D) B4GALT1, and (F) TGN46. Normalization was performed using β-actin. Quantification of relative total protein level of (C) SDF4, (E) B4GALT1, and (G) TGN46. Statistical analysis was done from three independent blots, where **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, * p ≤ 0.05. (H) (Left panel) Airyscan microscopy of RPE1 WT, VPS54KO, BET1LKO, and GOSR1KO cells stained for Rab6 and GM130. (Right panel) Quantification of trans-Golgi Rab6 area in WT, VPS54KO, BET1LKO, and GOSR1KO.