Skip to main content
. 2022 Dec 12;10:1066504. doi: 10.3389/fcell.2022.1066504

FIGURE 7.

FIGURE 7

GARP dysfunction resulted in mislocalization of COPI adapter protein GOLPH3 and ARFGAP1 from the Golgi. (A) Airyscan microscopy of VPS53KO, VPS53KO R, VPS54KO, VPS54KO R cells stained with GOLPH3 and GM130. (B) Colocalization analysis between GOLPH3 and GM130 using Pearson’s correlation coefficient. n = 40 cells were used for colocalization analysis. (C) VPS53KO, VPS53KO R, VPS54KO, and VPS54KO R cells were stained for ARFGAP1 and GM130. (D) Colocalization analysis of ARFGAP1 and GM130 using Pearson’s correlation coefficient. n= 40 cells were used for colocalization analysis. (E) WB analysis of ARFGAP1 total protein abundance in GARP-KO and control cells. (F) Quantification of total protein abundance of ARFGAP1 in GARP-KOs and control cells. β-actin was used as internal loading control. **** p ≤ 0.0001, ** p ≤ 0.01, * p ≤ 0.05.