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. 2022 Dec 12;10:1066504. doi: 10.3389/fcell.2022.1066504

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Copyright © 2022 Khakurel, Kudlyk, Pokrovskaya, D’Souza and Lupashin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ARFGEFs GBF1 and BIG1 are displaced to off-Golgi compartments in GARP-KO cells. (A) Airyscan microscopy of GARP-KO and control cells co-stained with antibodies to GBF1 and GM130 in RPE1 cells. (B) Colocalization analysis of GBF1 and GM130 in RPE1 cells. (C) WB analysis of GBF1 total protein abundance in GARP-KO and control cells. (D) Quantification of total protein abundance of GBF1 in GARP-KOs and control cells. β-actin was used as the internal loading control. (E) Colocalization analysis of GBF1 and GM130 in HeLa cells. (F) Colocalization analysis of GBF1 and GM130 in HEK293T cells. (G) Airyscan microscopy of GARP-KO and control cells co-stained with antibodies to BIG1 and GM130 in RPE1 cells. (H) Colocalization analysis of BIG1 and GM130 using Pearson’s correlation coefficient. n = 50 cells were used for colocalization analysis. **** p ≤ 0.0001, *** p ≤ 0.001, **p ≤ 0.01.