TABLE 1.
List of Antibodies used in the study.
| Antibody | Source/Catalog # | Species | WB dilution | IF dilution |
|---|---|---|---|---|
| Rab6 (C19) | Santa Cruz | Rabbit | — | 1:400 |
| #SC310 | ||||
| SDF4/Cab45 | Sigma HPA011249 | Rabbit | 1:1000 | 1:400 |
| ATP2C1 | Proteintech | Rabbit | 1:1000 | — |
| #I33I0-I-AP | ||||
| Golgin97 | Invitrogen | Mouse | — | 1:500 |
| #A21270 | ||||
| GM130 | BD Biosciences, 610823 | Mouse | — | 1:500 |
| GM130 | CalBiochem, CB1008 | Rabbit | — | 1:300 |
| β-actin | Sigma, A5441 | Mouse | 1:1000 | - |
| TGN46 | Bio-Rad, AHP500G | Sheep | 1:2000 | - |
| GOSR1/GS28 | BD Biosciences | Mouse | 1:500 | 1:500 |
| #611184 | ||||
| BET1L/GS15 | BD Biosciences | Mouse | - | 1:500 |
| #610961 | ||||
| B4GALT1 | R&D Systems, AF-3609 | Goat | 1:500 | - |
| COPB1 | Duden’s lab Duden et al. (1991) | Rabbit | 1:2000 | |
| COPB2 | ABclonal, A7036 | Rabbit | — | 1:400 |
| COPG1 | Duden’s lab | Rabbit | — | 1:2000 |
| ERGIC-53 | Enzo OTI1A8 | Mouse | — | 1:2000 |
| SNX1 | BD | Mouse | — | 1:300 |
| #S056 | ||||
| GBF1 | Sztul lab | Rabbit | 1:300 | |
| GBF1 | BD transduction laboratories | Mouse | — | 1:500 |
| #612116 | ||||
| BIG1(H-200) | Santa Cruz | Rabbit | — | 1:300 |
| #sc-50391 | ||||
| CD63 | DSHB, H5C6-C | Mouse | — | 1:200 |
| LAMP2 | DSHB, H4B4 | Mouse | 1:500 | - |
| GOLPH3 | Abcam | Rabbit | — | 1:500 |
| # ab98023 | ||||
| ARFGAP1 | ABclonal | Rabbit | 1:1000 | 1:500 |
| #A7118 | ||||
| IRDye 680 anti-Mouse | LiCOR/926–68170 | Goat | 1:40000 | - |
| IRDye 800 anti-Rabbit | LiCOR/926–32211 | Goat | 1:40000 | - |
| IRDye 800 anti-Goat | LiCOR/926–32214 | Donkey | 1:40000 | - |
| Alexa Fluor 647 anti-Rabbit | Jackson Immuno Research/711-605-152 | Donkey | 1:500 | 1:1000 |
| Alexa Fluor 647 anti-Mouse | Jackson Immuno Research/715-605-151 | Donkey | 1:500 | 1:1000 |
| Alexa Fluor 647 anti-Goat | Jackson Immuno Research/705-605-147 | Donkey | — | 1:1,000 |
| DyLight 647 anti-Sheep | Jackson Immuno Research/713-605-147 | Donkey | — | 1:1000 |
| anti-Rabbit Cy3 | Jackson Immuno Research/711-165-152 | Donkey | — | 1:1000 |
| anti-Mouse Cy3 | Jackson Immuno Research/715-165-151 | Donkey | — | 1:1000 |
| Alexa Fluor 488 anti-Rabbit | Jackson Immuno Research/711-545-152 | Donkey | — | 1:1000 |
| Alexa Fluor 488 anti-Mouse | Jackson Immuno Research/715-545-151 | Donkey | — | 1:1000 |