TABLE 1.

Intercellular matrix protein binding by a serotype M6 GAS strain and its isogenic rofA regulator mutant^a

Protein and strain type	Mean % dpm ± SD
	3 h	8 h	Overnight
Fibronectin
Wild type	54 ± 5	58 ± 8	54 ± 9
Mutant	38 ± 2	37 ± 6	33 ± 4
Fibrinogen
Wild type	52 ± 4	51 ± 3	53 ± 3
Mutant	45 ± 2	40 ± 3	44 ± 3
Collagen I
Wild type	24 ± 2	25 ± 1	31 ± 2
Mutant	24 ± 1	24 ± 1	31 ± 3

^aSoluble matrix proteins were ¹²⁵I labeled and employed for the binding assays as described by Kreikemeyer et al. (13). The bacteria were grown in Todd Hewitt-yeast extract broth to the early-logarithmic (3 h), early-stationary (8 h), and late-stationary (overnight) growth phases prior to the assays. The amount of radioactive material, measured in decays per minute (dpm), used for the assays was assigned a value of 100%; the amount of bound radioactive material (measured in dpm) was in turn related to these values. The table contains results from three independent assays.