Table 1.

Media names, components and functions within the context of this study.

Name	Components	Function
OB medium	DMEM low glucose 10 % FBS 1 % anti-anti 50 μg/mL L-ascorbic-acid-2-phosphate 100 nM dexamethasone 10 mM β-glycerophosphate	Osteogenic medium. One of three media compared during the co-culture. This medium was expected to further stimulate mineralized matrix deposition by OB.
Neutral medium	RPMI-1640 10 % FBS 1 % Anti-Anti	Unsupplemented medium. One of three media compared during the co-culture. This medium was expected to allow OB and OC crosstalk to control ongoing matrix deposition and resorption.
OC medium	RPMI-1640 10 % FBS 1 % Anti-Anti 50 ng/mL M-CSF 50 ng/mL RANKL	Osteoclastogenic medium. One of three media compared during the co-culture. This medium was expected to stimulate OC resorption.
MSC expansion medium	DMEM high glucose 10 % FBS 1 % anti-anti 1 % NEAA 1 ng/L bFGF	Used to expand MSCs prior to seeding onto scaffolds.
MSC seeding medium	DMEM high glucose 10 % FBS 1 % anti-anti	Unsupplemented medium that is used for MSC seeding onto scaffolds and prewetting of scaffolds.
Monocyte priming medium	RPMI-1640 10 % FBS 1 % Anti-Anti 50 ng/mL M-CSF	Used to prime monocytes during the first two days of culture which benefits osteoclastogenic differentiation.