Table 1.
Recombination activity of Int and some of its variants
| Recombinase | Substrate (positive reporter) |
|||
|---|---|---|---|---|
| attP/attB | COC’/COC’ | P-COC’/COC’ | COC’-P’/COC’ | |
| Int | <99% | ∼20% | ∼25% | <99% |
| Int(E174K) | <99% | <95% | <95% | <99% |
| Int(E174K, E218K) | <99% | <95% | ∼100% | ∼100% |
| C65 | 0% | 0% | nt | nt |
| C65(E174K) | 0% | ∼0.2% | nt | nt |
| C65(E174K, E218K) | 0% | ∼3% | nt | nt |
| C57 | 0% | 0% | nt | nt |
| C57(E174K) | 0% | ∼0.1% | nt | nt |
| C57(E174K, E218K) | 0% | ∼0.1% | nt | nt |
| [C65 + N64] | 0% | 0% | nt | nt |
| [C65(E174K) + N64] | 0% | ∼0.2% | nt | nt |
| [C65(E174K, E218K) + N64] | 0% | ∼5% | nt | nt |
| [C57 + N64] | 0% | 0% | nt | nt |
| [C57(E174K) + N64] | ∼2% | ∼0.1% | ∼0.1% | ∼10% |
| [C57(E174K, E218K) + N64] |
∼8% |
∼0.1% |
∼0.2% |
∼25% |
| Substrate (negative reporter) |
||||
|---|---|---|---|---|
| attP/attB | COC’/COC’ | P-COC’/COC’ | COC’-P’/COC’ | |
| Int | >99% | 0% | ∼0.1% | ∼99% |
| Int(E174K) | ∼97% | ∼50% | ∼50% | ∼95% |
| Int(E174K, E218K) | ∼95% | ∼90% | ∼70% | ∼95% |
| [C57(E174K) + N64] | ∼40% | 0% | 0% | ∼3% |
| [C57(E174K, E218K) + N64] | ∼70% | 0% | 0% | ∼10% |
nt, not tested.