REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC Rat Anti-Mouse CD11b (1:100)	SouthernBiotech	1561-11
PE-Cy7 Rat Anti-Mouse F4/80 (1:100)	BD Bioscience	#565410
SIRP-γ antibody (LSB2.20)	Santa Cruz	sc-53604
Ig κ chain antibody (L1C1)	Santa Cruz	sc-59265
Chemicals, peptides, and recombinant proteins
PMA	Absin	abs9107
M-CSF	Peprotech	AF-300-25
LPS	Sigma-Aldrich	297-473-0
IFN-γ	Peprotech	AF-315-05
Collagenase Type I	STEM CELL	#07415
Collagenase Type IV	STEM CELL	#07426
75% Ethyl Alcohol	CHRON CHEMICALS	64-17-5
Critical commercial assays
PKH26 Red Fluorescent Cell Linker MINI Kit	Sigma-Aldrich	MINI26
Genomic DNA Extraction Kit	TIANGEN	DP304
TB Green® Premix Ex Taq™ II	TaKaRa	RR820Q
BioTracker 488 CFSE	Sigma-Aldrich	SCT110
RPMI Medium 1640 basic (1×)	Gibco	C22400500BT
Fetal Bovine Serum	NEWZERUM	FBS-E500
Trypsin-EDTA (0.25%), phenol red	Gibco	25200072
Penicillin-streptomycin (10,000 U/mL) (P/S)	Gibco	#15140122
Red blood cell lysis buffer	Solarbio	R1010
Dulbecco’s modified Eagle’s medium (DMEM medium)	Gibco	C11995500BT
PBS, 1× (pH7.2–7.4, 0.01 M, cell culture)	Solarbio	P1020
Experimental models: Cell lines
A549 passage numbers: 5–20	ATCC	CCL-185™
NCI-H1975 passage numbers: 5–20	ATCC	CRL-5908™
THP1 passage numbers: 5–20	ATCC	TIB-202™
L-929 passage numbers: 5–20	ATCC	CCL-1™
NCI-H1975 shControl passage numbers: 5–20	Xu et al.1	N/A
NCI-H1975 shCD47 passage numbers: 5–20	Xu et al.1	N/A
Experimental models: Organisms/strains
Mouse: C57BL/6 mice, age 4–6 weeks, either gender	Beijing Huafukang Biological Technology Co., Ltd.	N/A
Mouse: Balb/c nude mice, age 4–6 weeks, either gender	Beijing Huafukang Biological Technology Co., Ltd.	N/A
Oligonucleotides
Primer: HUMAN PTGER2 Forward	Tsingke Biotechnology	GCTGCTTCTCATTGTCTCGG
Primer: HUMAN PTGER2 Reverse	Tsingke Biotechnology	CCAGGAGAATGAGGTGGTC
Primer: MOUSE PTGER2 Forward	Tsingke Biotechnology	CCTGCTGCTTATCGTGGCTG
Primer: MOUSE PTGER2 Reverse	Tsingke Biotechnology	GCCAGGAGAATGAGGTGGTC
Software and algorithms
GraphPad Prism	GraphPad	https://www.graphpad.com
Microsoft Excel	Microsoft	https://www.microsoft.com/zh-cn/microsoft-365/excel
Flowjo v10	BD Bioscience	https://www.flowjo.com
Other
70 μm cell strainers	Corning	#352350
0.45 μm sterile syringe filter	Sartorius	#16533
1 mL syringe with 26G needle	xjshifeng	SBI-080
15 mL centrifuge tubes	BIOFIL	CFT411150
50 mL centrifuge tubes	BIOFIL	CFT011500
100 mm Petri dish	NEST	704202
6-well plate	BIOFIL	TCP011006
24-well plate	BIOFIL	TCP011024
FACS tube	FALCON	352063
15 mm glass bottom cell culture dish	NEST	801002
Fuchs-Rosenthal Counting Chamber	MARIENFELD	AP0650030
Incubator	ESCO	CLM240B8CN
Sorvall Legend Micro 21R	Thermo Fisher	75002445
Soorvall ST 16R Centrifuge	Thermo Fisher	ST16R
CFX Connect Real-Time PCR Detection System	Bio-Rad	CFX96
FACS AriaII Flow cytometer	BD	N/A
Nikon A1R Confocal	Nikon	N/A