PCR reaction system
| Reagent | Usage amount |
|---|---|
| TB Green Premix Ex Taq II (2×) | 25 μL |
| PCR Forward Primer (10 μm) | 2.0 μL |
| PCR Reverse Primer (10 μm) | 2.0 μL |
| DNA | 200 ng (4 μL) |
| ddH2O | 17 μL |
| Total | 50 μL |
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