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. 2022 Dec 14;4(1):101938. doi: 10.1016/j.xpro.2022.101938

Immune cell culture media

Reagent Final concentration Amount
RPMI-1640 N/A 440 mL
HI-FBS 10% 50 mL
Penicillin-streptomycin (100×) 5 mL
HEPES (100×) 5 mL
Total N/A 500 mL