Reagent | Final concentration | Amount |
---|---|---|
RPMI-1640 | N/A | 440 mL |
HI-FBS | 10% | 50 mL |
Penicillin-streptomycin (100×) | 1× | 5 mL |
HEPES (100×) | 1× | 5 mL |
Total | N/A | 500 mL |
Reagent | Final concentration | Amount |
---|---|---|
RPMI-1640 | N/A | 440 mL |
HI-FBS | 10% | 50 mL |
Penicillin-streptomycin (100×) | 1× | 5 mL |
HEPES (100×) | 1× | 5 mL |
Total | N/A | 500 mL |