Figure 6.

Effect of complement inhibitors on killing of Hib isolate 23393 in reconstituted whole blood before and after ActHIB vaccination. Cumulated data for all study subjects are shown. The upper dashed lines corresponds to the amount of bacteria inoculated at time 0 (100% survival), while the lower dashed line represents the lower detection limit (< 100 CFU/mL) for bacterial quantification. Bacterial killing was assessed using PBS-washed EDTA-anticoagulated blood cells reconstituted with undiluted active serum, comparing serum taken before (A), 2 weeks (B) and 2 months (C) after ActHIB vaccination. CFUs were quantified 1, 3 and 5 hours after inoculation, represented by the 3 bars. The following inhibitor concentration were analyzed: fB inhibitor (iptacopan): 25 µM; fD inhibitor (CMS487): 25 µM; C3 inhibitor (CP-40): 50 µg/mL; anti-C5 mAb (tesidolumab): 50 µg/mL; and anti-MASP-2 mAb: 50 µg/mL.