Figure 2.
Interactions between T7 RNA polymerase and T7 promoter variants. (a) Measurements of the dissociation constants for T7 RNA polymerase and T7 promoter variants. The WT and variants of the T7 promoter sequence with additional linker nucleotides were biotinylated at the 5ʹ terminus of the sense strand DNA and immobilized on an SA chip as the ligand. The SPR experiments were performed by using a solution of T7 RNA polymerase as the analyte. (b) The impacts of the interactions between T7 RNA polymerase and T7 promoter variants. The dissociation equilibrium constant (KD) and the association rate constant (ka) for each promoter variant are shown. The averages are based on three independent replicates, and the error bars show standard deviations (SDs), which were calculated with the STDEVP function in Microsoft Excel.