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. 2022 Dec 16;33:101413. doi: 10.1016/j.bbrep.2022.101413

Fig. 4.

Fig. 4

Detection of anti-IA-2 autoantibodies in the serum of a subset of IA-2 positive type 1 diabetes patients using an MBP-IA-2 fusion protein (FP) ELISA. A. Representative MBP-IA-2 fusion protein ELISA binding using patient and control serum. Serum from a patient positive (T1D IA-2+) and negative (T1D IA-2-) for IA-2 autoantibodies by RSR commercial ELISA were assessed by MBP-IA-2 FP ELISA. Normalised OD values for the anti-IA-2 antibody positive patient of 0.58 exceeded the 0.198 cut off for positivity by MBP-IA-2 FP ELISA, as determined in Fig. 3. By comparison, the normalised OD of 0.078 for the T1D IA-2 – patient was below the cut off, as was value (0.026) for the healthy control serum (CTR). B. Nine individual sera from T1D patients positive for anti-IA-2 autoantibodies by RSR ELISA were then screened using the MPB-IA-2 FP ELISA. Of these, 3 (T1D019, T1D035 and T1D069) resulted in OD values above the assay cut off for positivity (dotted line). C. By comparison to the RSR positive patients, all 9 sera from RSR negative patients produced OD values by MBP-IA-2 ELISA below the assay cut off for positivity (dotted line). Values represent mean +SEM of at least n = 6 individual replicates. Each serum was assessed by MBP-IA-2 ELISA on at least 2 separate occasions.