Table 2.
Establishment of cut off values for positivity by IA2/MBP FP and ZnT8/MBP FP ELISAs.
| CTR | Mean OD | SD |
|---|---|---|
| 1 | 0.0195 (0.0318) | 0.0212 (0.0025) |
| 2 | 0.0903 (0.0985) | 0.0470 (0.0608) |
| 3 | 0.08 (0.0255) | 0.0071 (0.0099) |
| 4 | 0.0238 (0.145) | 0.0166 (0.0219) |
| 5 | 0.1333 (0.0065) | 0.0124 (0.0085) |
| 6 | 0.0495 (0.1295) | 0.0113 (0.0191) |
| 7 | 0.0325 (0.0465) | 0.0127 (0.000) |
| 8 | 0.074 (0.0630) | 0.0453 (0.0127) |
| 9 | 0.109 (0.018) | 0.0523 (0.0106) |
| 10 | 0.0213 (0.0295) | 0.0301 (0.0347) |
| Pooled CTRs |
0.0634 (0.0594) |
0.045 (0.0486) |
| OD value cut off for positivity | ||
| IA-2 FP ELISA 0.198 | ||
| ZnT8 FP ELISA 0.194 | ||
*Values are mean and SD of n = 4 replicates for individual control (CTR 1–10) serums assessed on IA-2/MBP (unbracketed) and ZnT8/MBP (bracketed) FP ELISAs. Pooled values and SDs were used to generate positivity cut off scores representing the mean + 3 SDs.