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(a) ROS detection by flow cytometry with HK-2 cells at 24 h (n = 5). (b) CCK-8 assay at 24 h (n = 5). (c) (d) The apoptosis ratio was assessed with HK-2 by flow cytometry statistical analysis at 24 h (n = 5). (e) ROS detection by fluorescence microscopy at 24 h (n = 5). Scale bars = 100 μm. (f) The apoptosis ratio was assessed with HK-2 by fluorescence microscopy at 24 h (n = 5). Scale bars = 100 μm. The data are presented as the mean ± SD ∗∗P < 0.01, ∗∗∗P < 0.001.
