Skip to main content
. 2022 Dec 13;8(12):e12269. doi: 10.1016/j.heliyon.2022.e12269

Figure 5.

Figure 5

(a) (b) TUNEL fluorescence staining of proximal tubular epithelial cells in the kidney cortex and statistical analysis of cell death rates in each group (n = 5). Green fluorescence in the pictures represents positive signals (cell death), while blue fluorescence represents nuclear staining. Scale bars = 100 μm. (c) (d) Expression and location of Cytochrome C and Cleaved Caspase 3 by immunohistochemistry, followed by semiquantitative statistical analysis of each group (n = 5). Scale bar = 100 μm. (e) (f) Protein expression levels of Bax, Bcl-2, Cytochrome C, and Cleaved-Caspase 3 by Western blot, followed by semiquantitative statistical analysis of each group (n = 5). (g) Detection of mRNA levels of Bax, Bcl-2, Cytochrome C, and Cleaved-Caspase 3 by quantitative real-time PCR (n = 5). The data are presented as the mean ± SD ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. Cyto-C, Cytochrome C; C- Caspase 3, Cleaved Caspase 3. The uncropped images of (e) were referred to in Supplementary Full non-adjusted Figure.