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EPEC 30-5-1(3) is attenuated in forming EspA filaments. DMEM-grown bacteria were stained with rabbit anti-EspA followed by an anti-rabbit antibody conjugated with FITC. (A and B) Immunofluorescence and corresponding phase image for wild-type EPEC, respectively; (C and D) immunofluorescence and corresponding phase image for EPEC 30-5-1(3), respectively.
