(A) Microglial-cell-enriched cultures, 14 days after inoculation with B. henselae, display evidence of infection based on positive Giménez staining for bacteria. Bacterial aggregates are identified in the perinuclear region of the cells (arrows). (B) Astrocyte-enriched cultures, 14 days after inoculation with B. henselae, reveal no evidence of cellular infection when stained by the Giménez method. Bars, 25 μm.