FIG. 2.
Epitope-mapping analyses of neutralizing MAbs by SPR. A biosensor chip with immobilized anti-mouse Fc was equilibrated with HBS buffer, and the following solutions were passed sequentially over the chip with a 2-min injection of HBS buffer between each reagent: MAb 4A2-2 for 3 min (a), unrelated mouse MAb for 3 min (to block all remaining anti-mouse Fc sites) (b), 10 μg of antigen BoNT/A HC per ml for 3 min (c), and the second MAb for 3 min, either 4A2-2, 6B2-2, 6E9-1, or 6E10-10 as indicated (d). There is a response, sometimes large, at each injection due to the change in the refractive index of the medium.