TABLE 1.
Specificity of BoNT/A neutralizing MAbs
| MAb | Isotypea | BoNT serotype | ELISA resultb | LD50c | No. live/ no. deadd |
|---|---|---|---|---|---|
| 4A2-2 | IgG2a | A | +++ | 10 | 5/0 |
| B | ND | 5 | 0/5 | ||
| E | ND | 5 | 0/5 | ||
| 4A2-4 | IgG2a | A | +++ | 10 | 0/5 |
| 6B2-2 | IgG1 | A | +++ | 10 | 5/0 |
| B | ND | 5 | 0/5 | ||
| E | ND | 5 | 0/5 | ||
| 6C2-4 | IgG1 | A | +++ | 10 | 5/0 |
| B | ND | 5 | 0/5 | ||
| E | ND | 5 | 0/5 | ||
| 6E9-1 | IgG2a | A | +++ | 10 | 5/0 |
| B | ND | 5 | 0/5 | ||
| E | ND | 5 | 0/5 | ||
| 6E9-3 | IgG2a | A | +++ | 10 | 5/0 |
| 6E9-4 | IgG2a | A | +++ | 10 | 5/0 |
| 6E10-4 | IgG2a | A | +++ | 10 | 5/0 |
| 6E10-5 | IgG2a | A | +++ | 10 | 5/0 |
| 6E10-8 | IgG2a | A | +++ | 10 | 5/0 |
| 6E10-10 | IgG2a | A | +++ | 10 | 5/0 |
a
The isotypes were determined as described in the text.
b
ELISA wells were coated with BoNT/A, BoNT/B, or BoNT/E. The MAbs were added to the wells at 100 ng/well, and bound antibodies were detected with horseradish peroxidase-labeled antibody. OD readings above 1.2 are presented as +++, while OD readings below 0.05 are shown as ND (not detected).
c
The antibody was incubated for 1 h with 10 LD50 of BoNT/A or 5 LD50 of BoNT/B or BoNT/E before being administered to the mice. Lethality was recorded 7 days after the challenge dose.
d
Final lethality was statistically scored using Fisher exact tests. P < 0.001 indicates protection against BoNT/A versus BoNT/B or BoNT/E.