TABLE 2.

Kinetic constants of BoNT/A MAbs binding to BoNT/A H_C^a

MAb	RUb of MAb	koff (M−1 s−1)	kon (s−1)	KD (nM)
4A2-2	150	3.5 × 10−4	6.9 × 105	0.51
4A2-4	128	4.2 × 10−4	7.8 × 105	0.54
6B2-2	263	<0.2 × 10−4	3.1 × 105	<0.06
6C2-4	148	1.9 × 10−4	17 × 105	0.11
6E9-1	170	2.5 × 10−4	11 × 105	0.22
6E9-3	121	2.8 × 10−4	11 × 105	0.25
6E9-4	104	6.7 × 10−4	7.8 × 105	0.86
6E10-4	80	5.3 × 10−4	11 × 105	0.48
6E10-5	128	3.9 × 10−4	4.8 × 105	0.81
6E10-8	124	2.1 × 10−4	4.9 × 105	0.42
6E10-10	90	3.1 × 10−4	14 × 105	0.22

^aThe kinetic constants were measured by SPR as described in the text. 

^bThe amount of MAb captured by the chip-immobilized anti-mouse Fc is shown as RU (response units).