Figure 5.

HKL improved the barrier function of the DSS-induced mice. (a) The levels of ZO1, occludin, and Claudin1 mRNA were measured by qRT-PCR analyses. mRNA expression was normalized to β-actin expression. (b) Western blots and related quantification of ZO1, occludin, and Claudin1 are expressed as a percent of control. (c) Immunohistochemistry analysis of the expression of MUC2, MUC3, and TFF3 (×200); the black arrows mark the protein. Data are expressed as mean ± SEM (n = 3). ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001.