Table 1.

Immunization procedure

Groups	Immunization dosages	Challenge (63 days of age)
Unchallenged control	1 ml PBS	/
Challenged control	1 ml PBS	1 × 105 E. magna
Adjuvant control	1 mg saponin	1 × 105 E. magna
Vector protein	100 μg pET-32a( +) + 1 mg saponin	1 × 105 E. magna
Recombinant EmMIC2	100 μg rEmMIC2 + 1 mg saponin	1 × 105 E. magna
Recombinant EmMIC3	100 μg rEmMIC3 + 1 mg saponin	1 × 105 E. magna

Immunization was administered subcutaneously in the neck, with a total volume of 1 mL