Table 2.
Additional annotation and gene expression data for 19 prioritized biomarker candidate proteins
| Protein ID | Protein functional annotation | Protein length (AA) | Predicted secretion | Gene exp. Level (avg. adult female FPKM) | Gene expression consistency (% of adult females) | Missense + nonsense SNP rate (Choi et al, 2016) |
|---|---|---|---|---|---|---|
| OVOC11613 | Major antigen/lfi-1 - Lin-5 (Five) Interacting | 2021 | − | 17.0 | 100% | 3.6 |
| OVOC1523 | ATP synthase subunit alpha, mitochondrial | 535 | − | 1963.9 | 100% | 1.2 |
| OVOC247 | Ovo-lam-3 - Laminin alpha chain | 3358 | Signal peptide | 24.9 | 100% | 2.7 |
| OVOC11626 | Inactive serine/threonine-protein kinase PLK5 | 268 | Nonclassical | 2.0 | 100% | 11.2 |
| OVOC9912 | IPR003609:PAN/Apple domain | 628 | Signal peptide | 0.3 | 87.5% | 15.4 |
| OVOC4989 | Ovo-unc-22 - Twitchin homolog | 6785 | − | 28.9 | 100% | 2.3 |
| OVOC10067 | Basement membrane proteoglycan | 3415 | Signal peptide | 0.9 | 100% | 7.1 |
| OVOC2745 | Actin-4 | 376 | − | 359.1 | 100% | 0.9 |
| OVOC7004 | Putative cubilin | 4112 | Signal peptide | 0.0 | 12.5% | 22.0 |
| OVOC7893 | H2B: histone H2B | 380 | − | 72.2 | 100% | 2.6 |
| OVOC10971 | Ovo-phb-2 - Mitochondrial prohibitin complex protein 2 | 568 | − | 275.7 | 100% | 4.1 |
| OVOC1599 | Ovo-adpr-1 - WDTC1, WD and tetratricopeptide repeats | 597 | − | 21.8 | 100% | 0.6 |
| OVOC7471 | Ovo-magu-2 - MAGUK p55 subfamily member 5 | 861 | − | 0.6 | 100% | 8.1 |
| OVOC216 | still life protein homolog | 987 | − | 12.8 | 100% | 1.7 |
| OVOC1112 | Ovo-smg-1 - Serine/threonine-protein kinase smg-1 | 2527 | − | 13.8 | 100% | 3.8 |
| OVOC3364 | (Filarial-specific) | 166 | Signal peptide | 0.0 | 38% | 12.5 |
| OVOC874 | (Nematode-specific) | 239 | − | 106.1 | 100.0% | 0.0 |
| OVOC8003 | Ovo-lgc-50 - Ligand-gated ion channel 50 homolog | 221 | − | 0.1 | 63% | 8.7 |
| OVOC9445 | PPP1C: serine/threonine phosphatase PP1 catalytic | 671 | − | 9.8 | 100.0% | 0.0 |
Included are the protein lengths (amino acids), predicted secretion, gene expression in adult females (RNA-seq), gene expression consistency among adult females, and the rate of missense + nonsense single nucleotide polymorphisms (SNPs) per kilobase of protein sequence, based on results from Choi et al, 2016 (46).
Abbreviation: FPKM, fragments per kilobase per million reads.