FIGURE 2.

XFZYD pretreatment promoted neurological protection after tMCAO surgery. (A–B) Neurological deficit scores and TTC staining were measured 24 h after surgery. (A) Comparison of neurological deficit scores in tMCAO and XFZYD groups (n = 18 in each group, ^## p < 0.01 vs. the tMCAO group). (B) Representative brain sections stained with TTC are shown and infarct volume was quantified and compared between the XFZYD and tMCAO groups (n = 6 in each group, ^# p < 0.05 vs. the tMCAO group). (C) Representative photomicrographs of HE staining (Scale bar, 50 μm). Nerve cells in the infarcted area had incurred damaged, as demonstrated by nucleolus pyknosis (red arrow), fragmentation (yellow arrow), nucleolus dissolution (green arrow) and vacuolated spaces (blue arrow).