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. 2022 Dec 13;13:1053253. doi: 10.3389/fphar.2022.1053253

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Copyright © 2022 Yi, Gao, Hou, Lv, Huang, Gao, He, Yang, Chen, Zhu, Yu, Liu, Yin and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of tMCAO and XFZYD on the colocalization of AQP4, α-DG and β-DG. (A) The immunostaining of AQP4, α-DG and β-DG in the infarct and periinfarct areas (Scale bars, 200μm and 500 μm). (B) Quantitative analysis of data A in infarct area and periinfarct area. (C) Representative colocalization images of AQP4, α-DG and β-DG immunostaining in the meningeal (α-DG, left; β-DG, right, Scale bar, 10 μm). (D) Quantification of the colocalization of AQP4, α-DG and β-DG by Manders’ tM1, Manders’ tM2 and Pearson’s R value in different groups. (^* p < 0.05, ^** p < 0.01, ^*** p < 0.001 vs. the sham group, ^# p < 0.05, ^### p < 0.001 vs. the tMCAO group; Data represent mean ± SD, n = 6).