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. 2022 Dec 13;13:1053253. doi: 10.3389/fphar.2022.1053253

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Copyright © 2022 Yi, Gao, Hou, Lv, Huang, Gao, He, Yang, Chen, Zhu, Yu, Liu, Yin and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of tMCAO and XFZYD on the colocalization of AQP4 and agrin. (A) The immunostaining of AQP4 and agrin in the three groups (Scale bar, 1 mm). (B) Quantitative analysis of data A in the infarct and periinfarct areas. (C) Representative colocalization images of agrin and AQP4 immunostaining in the meningeal (Scale bar, 20 μm) (D) Quantification of the colocalization of AQP4 and agrin by Manders’ tM1, Manders’ tM2 and Pearson’s R value from different groups in the meningeal (*p < 0.05, **p < 0.01 vs. the sham group, ^# p < 0.05 vs. the tMCAO group; Data represent mean ± SD, n = 6).