FIGURE 3.
MDM2 inhibitors activate p53 pathway in alveolar basal epithelial cells. Western blotting analysis of p53, MDM2 and Rb proteins in A549-hACE2 cell cultures treated with different concentrations of Nutlin-3 (A,C,E) or RG-7112 (B,D,F) and relative controls (Unt, untreated; DMSO, control vehicle). Protein levels were evaluated 24 h after treatments (A,B) and 48 h after SARS-CoV-2 infection (corresponding to 72 h after initial treatment) in infected (C,D) and mock cultures (E,F). Images are representative of three independent experiments. β-tubulin is used as internal control.