FIGURE 4.

Quantification of SARS-CoV-2 infection. Quantification of SARS-CoV-2 genomes determined by RT-qPCR in 25 µL A549-hACE2 supernatants collected at 48 h post-infection of cultures treated with different concentrations of Nutlin-3 (A) or RG-7112 (B) and relative controls (Unt, untreated; DMSO, control vehicle). Results were plotted as mean ± standard error of the mean of two independent experiments run in triplicates. Statistical analysis was performed by Student’s t test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.