FIGURE 5.

Quantification of inflammatory environment. Relative quantification (RQ) of expression levels of IL-6 (A,B) and IFN-λ1 (C,D) and NF-κB (E,F), in SARS-CoV-2-infected A549-hACE2 cells treated with different concentrations of Nutlin-3 (A,C,E) or RG-7112 (B,D,F) and relative controls (Unt, untreated; DMSO, control vehicle). Gene expression was normalized to GAPDH housekeeping gene, and quantification is relative to not infected untreated cells. Mean ± standard error of the mean of two independent experiments in triplicate are shown. Statistical analysis was performed by Student’s t test. *p ≤ 0.05; **p ≤ 0.01.