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Two-dimensional nonequilibrium gel electrophoresis of Triton X-100-solubilized proteins from 4 × 108 B. burgdorferi of strains B31, N40, 297, and Sh-2-82. (A) Amido black stain. (B) Immunoblot in panel A probed with pooled B31 infection-immune prechallenge sera. Molecular size markers are indicated to the left (in kilodaltons). Acidic and basic ends are indicated at the top.
