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. 2022 Dec 13;9:1036757. doi: 10.3389/fvets.2022.1036757

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Copyright © 2022 Parvin, Kabiraj, Hossain, Hassan, Begum, Nooruzzaman, Islam and Chowdhury.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RT-qPCRs amplification plots for specific target viral pathogens. (A) Simultaneous detection of generic avian influenza virus (AIV-M) and subtyping of AIV (H5, H9, N1, and N2) in a single run. (B) Simultaneous detection of Newcastle disease virus (ND), infectious bronchitis virus (IBV), and infectious laryngotracheitis virus (ILT) along with the melt curve (C) in a single run. NC denotes a negative control that shows a melt curve at a different temperature (~75°C). The Ct values of individual gene is indicated by dash lines (A,B) generated from the number of cycles.