Table 2.
Detection limit (Ct) and reproducibility of reference samples in applied multitarget simultaneous RT-qPCR assays.
| Reference samples | Single target RT-qPCRs | Multitarget simultaneous RT-qPCRs | ||
|---|---|---|---|---|
| Average Cta | S.E.Mb | Average Cta | S.E.Mb | |
| AIV (1–10) | 21.2 | ±0.13 | 21.5 | ±0.12 |
| NDV (1–10) | 25.5 | ±0.24 | 25.4 | ±0.27 |
| IBV (1–10) | 23.2 | ±0.18 | 23.3 | ±0.15 |
| ILTV (1–5) | 24.4 | ±0.19 | 24.0 | ±0.18 |
| Negativec (1–10) | Undetermined | N/A | Undetermined | N/A |
a
Average Ct value from triplicate independent single-target and multitarget simultaneous assay runs.
b
Standard error of the average Ct values.
c
Known negative for AIV, NDV, IBV, and ILTV.