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. 2022 Dec 27;12:22402. doi: 10.1038/s41598-022-26650-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NanoString GeoMx spatial protein and transcriptomic profiling: (A) Image of a lung tissue section from a representative COPD subject stained with pan-cytokeratin (green), CD45 (red) and SYTO13 (blue). The regions of interest (ROIs) sampled for spatial protein and RNA analysis are highlighted in the white boxes. (B) The workflow of protein and RNA data analyses is shown.