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. 2022 Dec 16;4(1):101953. doi: 10.1016/j.xpro.2022.101953

Figure 4.

Figure 4

Purification and analysis of hPSC-derived hematopoietic stem and progenitor cells

(A) Schematic of the protocol for the purification of hPSC-derived hematopoietic stem and progenitor cells.

(B) Representative flow cytometry plots of CD45 expression before and after purification.

(C) Representative flow cytometry plots of CD43 and RUNX1c expression on the purified definitive hematopoietic cells derived from H9 Sox17-mCherry RUNX1c-GFP dual reporter.

(D) Representative histogram plot of CD34 expression on the purified definitive hematopoietic cells.