FIG. 1.

Time course of IL-8 mRNA induction in undifferentiated HT29-18N2 colonic epithelial cells by reactogenic and nonreactogenic cholera vaccine strains. Data shown are from a representative gel electrophoresis of three independent RT-PCR amplification products of β-actin and IL-8 mRNAs from undifferentiated HT29-18N2 epithelial cells, after stimulation with four V. cholerae vaccine strains at various time intervals from 0 to 8 h. Numbers under each panel represent densitometry values and are expressed in arbitrary units. Sizes are indicated in base pairs (bp).