FIG. 2.
Dose-dependency experiments for IL-8 mRNA induction in undifferentiated HT29-18N2 colonic epithelial cells by reactogenic and nonreactogenic cholera vaccine strains. Data shown are from a representative gel electrophoresis of three independent RT-PCR amplification products of β-actin and IL-8 mRNAs from undifferentiated HT29-18N2 epithelial cells, after 4 h of stimulation with two V. cholerae vaccine strains; inoculum size ranged from 104 to 107 CFU/well. Numbers under each panel represent densitometry values and are expressed as arbitrary units. Sizes are indicated in base pairs (bp).